Experiment+13

Isolation of Genomic DNA and Ligation into pBluescript SK+

-given Streptococcus pnuemoniae -lyse by heating in SDS and proteinase(protease?) -extract nucleic acids w/ chloroform and isoamyl alcohol. -Genomic DNA will be recovered by precipitation -Precipitate with ethanol(95%) wash w/70% -redissolve in TE buffer -restrict with ECOR1 -ligate into pBluescript SK+ -amplify in e.coli